Nikita, please use this page as your science notebook for your summer internship – all your answers, observations, questions, and results – all should be added here on this page (you can add text and post pictures here; using this page will also teach you how to use the WordPress platform). Please start with internet research regarding the instrument you received by answering the following questions (copy/paste will not work, you will need to perform the internet research using google.com and then write your own answers to the following questions):
Model and name of the instrument: The method of H&E staining is used to stain different parts of the cell so that scientists can see what the cell look like in greater detail
What is it used for? It is used to find what cells and tissues look like
Who needs it? The people who need it are the scientists who want to find out what cells or tissues look like
What parts is it made of? It has 2 parts to it, the first part is the Hematoxylin which stains the nuclei of the cell blue, and the Eosin stains the cytoplasm pink. Other organelles are stained in a mixture of those colors.
How does it work? Describe in great detail. The first step in the H&E staining method is the gathering of the tissues, after that they are dehydrated and put into slides, after they are put into slides they are dipped into the solution.
Were you able to find an instrument manual online? Although I did’t find a manual, I was able to use other sources such as Wikipedia to gather information.
Read the manual. Describe what was clear in the manual and what was confusing. N/A
After you have completed internet research, try to turn on the instrument and see what happens. Make a picture and post it here. You may be missing power cord and/or adapter cables (find out what cords/cables/adapters are missing, find missing part on Amazon or Ebay, and post Amazon/Ebay link here, so I can get you the missing part). Some instruments been in storage and are very dusty – clean them with Windex spray and paper towels (your parents got a cleaning supplies cabinet somewhere in your house, find it).
Try to operate the instrument using the manual … is it working? Do you need certain supplies to make it work? Find the catalog numbers (from the manual) of the required supplies, where from they are available and post here links of what we need to order. Post here results of your testing, and how do you plan to move forward.
Read the details on what is Histology, how H&E works, how to identify different type of cells and tissue (how different disease cells/tissues look?):
A Beginner’s Guide to Haematoxylin and Eosin Staining
https://histology.leeds.ac.uk/what-is-histology/H_and_E.php
After you have completed internet research, try to turn on the instrument and see what happens. Make a picture and post it here. You may be missing power cord and/or adapter cables (find out what cords/cables/adapters are missing, find missing part on Amazon or Ebay, and post Amazon/Ebay link here, so I can get you the missing part). Some instruments been in storage and are very dusty – clean them with Windex spray and paper towels (your parents got a cleaning supplies cabinet somewhere in your house, find it).
Try to operate the instrument using the manual … is it working? Do you need certain supplies to make it work? Find the catalog numbers (from the manual) of the required supplies, where from they are available and post here links of what we need to order. Post here results of your testing, and how do you plan to move forward.
This is your notebook now … write and post answers and pictures below:
Nikita:
From Dima: 6/30/2020 update: Nikita, please post pictures and and text here so I can see your progress.
7/26/2020: From Dima: Internship deadline is August 17, 2020. You have 3 weeks left, please note that it will not be possible to add text/data after this date due to the nature of summer internship and associated time limits.
8/15/2020: Internship was not completed by 8/15/2020.